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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VIANCELLI, A.; PRA, M. C. de; SCUSSIATO, L. A.; CANTAO, M. E.; IBELLI, A. M. G.; KUNZ, A. |
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Afiliação: |
ALINE VIANCELLI, UNC/Concórdia; MARINA CELANT DE PRÁ; LUCAS ANTUNES SCUSSIATO, UNIOESTE/Cascavel; MAURICIO EGIDIO CANTAO, CNPSA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; AIRTON KUNZ, CNPSA. |
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Título: |
Preservation and reactivation of candidatus jettenia asiatica and anammoxoglobus propionicus using different preservative agents. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Chemosphere, v. 186, p. 453-458, 2017. |
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DOI: |
10.1016/j.chemosphere.2017.07.053 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation with glycerol at 80 C was successful for Candidatus Jettenia asiatica. The present study was effective on anammox sludge preservation and reactivation using low-cost processes for anammox cultures preservation, which is important for biomass transport and deammonification reactor start up. © 2017 MenosAbstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation ... Mostrar Tudo |
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Palavras-Chave: |
Anammox; Gestão ambiental; Lodo. |
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Thesagro: |
Meio ambiente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02526naa a2200241 a 4500
001 2081303
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.chemosphere.2017.07.053$2DOI
100 1 $aVIANCELLI, A.
245 $aPreservation and reactivation of candidatus jettenia asiatica and anammoxoglobus propionicus using different preservative agents.$h[electronic resource]
260 $c2017
520 $aAbstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation with glycerol at 80 C was successful for Candidatus Jettenia asiatica. The present study was effective on anammox sludge preservation and reactivation using low-cost processes for anammox cultures preservation, which is important for biomass transport and deammonification reactor start up. © 2017
650 $aMeio ambiente
653 $aAnammox
653 $aGestão ambiental
653 $aLodo
700 1 $aPRA, M. C. de
700 1 $aSCUSSIATO, L. A.
700 1 $aCANTAO, M. E.
700 1 $aIBELLI, A. M. G.
700 1 $aKUNZ, A.
773 $tChemosphere$gv. 186, p. 453-458, 2017.
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